Abts Metodo

ABTS radical cation ABTS was produced by reacting ABTS stock solution with 245 mM potassium persulfate final concentration and allowing the mixture to stand in the dark at room temperature for 1216 h before use. In the ABTS assay also known as Trolox equivalent antioxidant capacity TEAC assay the greenblue stable radical cationic chromophore 22-azinobis-3-ethylbenzothiazoline-6-sulfonate ABTS.

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Pütter Becker 1983The radical formation is catalyzed by the reduction of HRP in the presence of hydrogen peroxide Fig.

Abts metodo. The ABTS assay is a colorimetric assay based on the ABTS cation radical formation Keesey 1987. ABTS was dissolved in water to a 7 mM concentration.

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